Table 2.
Bacterial strains and plasmids used in this study.
| Strain/Plasmid | Markers/Description | Reference/Source |
|---|---|---|
| Escherichia coli One Shot TOP 10 | F-mcrA Δ (mrr-hsdRMS-mcrBC) Φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara-leu)7697 galU galK rpsL StrR endA1 nupG | Invitrogen |
| Escherichia coli S17-1 | recA pro hsdR RP4-2-Tc:Mu-Km:Tn7 TmpR SpcRStrR | [28] |
| pCR2.1 | KanR, AmpR | Invitrogen |
| pDrive | KanR, AmpR | Qiagen |
| pCM184 | Broad-host-range allelic exchange vector, KanR, TetR | [27] |
| pCM157 | Cre/LoxP, TetR | [27] |
| pMgdD | pCM184 with mgdD upstream and downstream flanks | This study |
| pMgsC | pCM184 with mgsC upstream and downstream flanks | This study |
| pFAE1 | pCM184 with fae1 upstream and downstream flanks | This study |
| pFAE2 | pCM184 with fae2 upstream and downstream flanks | This study |
| pFAE3 | pCM184 with fae3 upstream and downstream flanks | This study |
| pQHNDH1 | pCM184 with qhpA upstream and downstream flanks | This study |
| pQHNDH2 | pCM184 with qhpX upstream and downstream flanks | This study |
| pMtdA | pCM184 with mtdA upstream and downstream flanks | This study |