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. 2015 Apr 9;3(2):175–197. doi: 10.3390/microorganisms3020175

Table 3.

Gene expression profile in methane or methylamine grown cells of M. universalis FAM5.

Gene ID (Old) Gene ID (New) Gene Product Methanol (RPKM) * Methylamine (RPKM) * Fold Change q-Value
Methylamine oxidation
METUNv1_760110 METUNv2_580110 Gmas, Gamma glutamylmethylamide synthetase 2961.80 19,440.25 6.56 0.65
METUNv1_760111 METUNv2_580111 MgsA, N-methyl glutamate synthase subunit A 2727.43 22,221.30 8.15 0.73
METUNv1_760112 METUNv2_580112 MgsB, N-methyl glutamate synthase subunit B 2680.76 19,852.55 7.41 0.69
METUNv1_760113 METUNv2_580113 MgsC, N-methyl glutamate synthase subunit C 4221.56 53,956.55 12.78 0.67
METUNv1_760114 METUNv2_580114 MgdA, N-methyl glutamate dehydrogenase/oxidoreductase subunit A 1414.90 13,014.45 9.20 0.74
METUNv1_760115 METUNv2_580115 MgdB, N-methyl glutamate dehydrogenase/oxidoreductase subunit B 388.00 3906.31 10.07 0.74
METUNv1_760116 METUNv2_580116 MgdC, N-methyl glutamate dehydrogenase/oxidoreductase subunit C 3389.10 26,611.05 7.85 0.75
METUNv1_760117 METUNv2_580117 MgdD, N-methyl glutamate dehydrogenase/oxidoreductase subunit D 701.94 7962.74 11.34 0.71
METUNv1_760127 METUNv2_580127 QhpB, Quinohemoprotein amine dehydrogenase, beta subunit 420.06 4164.18 9.91 0.68
METUNv1_760128 METUNv2_580128 QhpA, Quinohemoprotein amine dehydrogenase, alpha subunit 733.76 7390.84 10.07 0.65
METUNv1_760130 METUNv2_580130 QhpC, Quinohemoprotein amine dehydrogenase, SAM-radical dependent activating subunit 380.86 4283.06 11.25 0.00
Methanol oxidation
METUNv1_770214 METUNv2_590217 XoxF1, PQQ-linked dehydrogenase 2023.28 3380.16 1.67 0.50
METUNv1_770216 METUNv2_590218 XoxF2, PQQ-linked dehydrogenase 10,962.17 16,741.70 1.53 0.05
METUNv1_590046 METUNv2_420045 XoxF3, PQQ-dependent dehydrogenase 1213.63 659.01 −1.84 0.62
METUNv1_590042 METUNv2_420041 XoxJ3, Extracellular solute-binding protein family 3 4399.57 1403.51 −3.13 0.05
METUNv1_590043 METUNv2_420042 XoxG3, Cytochrome c class I 3156.11 1089.89 −2.90 0.30
METUNv1_590049 METUNv2_420048 Mdh2, PQQ-dependent methanol/ethanol dehydrogenase 48,100.35 34,793.20 −1.38 0.77
METUNv1_590050 METUNv2_420049 Mdh2J, Extracellular solute-binding protein family 2486.88 903.00 −2.75 0.69
METUNv1_590051 METUNv2_420050 Mdh2G, cytochrome c-type protein 1732.91 595.39 −2.91 0.58
Formaldehyde oxidation
METUNv1_580096 METUNv2_410093 Fae 1, Formaldehyde-activating enzyme 18,235.00 11,635.85 −1.57 0.82
METUNv1_660037 METUNv2_480039 Fae 2, Formaldehyde-activating enzyme 561.52 642.50 1.14 0.89
METUNv1_700516 METUNv2_520523 Fae 3, Formaldehyde activating enzyme 584.51 6861.86 11.74 0.00
METUNv1_590006 METUNv2_420006 Orf 9, Involved in biosynthesis of tetrahydromethanopterin. Essential for formaldehyde oxidation. 430.06 187.51 −2.29 0.21
METUNv1_580095 METUNv2_410092 Orf 7, Involved in tetrahydromethanopterin-linked formaldehyde oxidation. 371.25 549.26 1.48 0.75
METUNv1_580094 METUNv2_410091 Orf 5, Involved in biosynthesis of tetrahydromethanopterin 489.69 493.89 1.01 0.93
METUNv1_580093 METUNv2_410090 Mch, Methenyltetrahydromethanopterin cyclohydrolase 620.26 736.89 1.19 0.87
METUNv1_580092 METUNv2_410089 OrfY, Involved in tetrahydromethanopterin C1 transfer. 896.40 819.12 −1.09 0.94
METUNv1_580091 METUNv2_410088 MtdB, NAD-dependent methylenetetrahydromethanopterin dehydrogenase 1295.29 1379.77 1.07 0.88
METUNv1_580088 METUNv2_410086 FhcB, Formyltransferase/hydrolase complex subunit B 587.63 515.00 −1.14 0.92
METUNv1_580087 METUNv2_410085 FhcA, Formyltransferase/hydrolase complex subunit A 766.77 744.38 −1.03 0.93
METUNv1_580086 METUNv2_410084 FhcD, Formyltransferase/hydrolase complex subunit D 598.76 547.64 −1.09 0.94
METUNv1_580085 METUNv2_410083 FhcC, Formyltransferase/hydrolase complex subunit C 634.50 467.32 −1.36 0.88
METUNv1_590040 METUNv2_420039 Ald, Tungsten-containing aldehyde ferredoxin oxidoreductase 408.13 155.57 −2.62 0.17
METUNv1_490013 METUNv2_320014 AldB, Aldehyde dehydrogenase B 2762.93 2224.63 −1.24 0.94
Formate oxidation
METUNv1_770385 METUNv2_590384 Fdh, Putative formate dehydrogenase subunit A 634.02 668.94 1.06 0.92
METUNv1_700257 METUNv2_520260 FdhD, NAD-linked formate dehydrogenase delta subunit 38.00 76.63 2.02 0.55
METUNv1_700258 METUNv2_520261 FdhC, Formate dehydrogenase, accessory protein 751.50 1024.07 1.36 0.74
METUNv1_700259 METUNv2_520262 FdhA, NAD-dependent formate dehydrogenase alpha subunit 3720.44 5479.72 1.47 0.39
METUNv1_700260 METUNv2_520263 FdhB, NAD-dependent formate dehydrogenase beta subunit 2758.00 4434.84 1.61 0.46
METUNv1_700261 METUNv2_520264 FdhG, NAD-dependent formate dehydrogenase gamma subunit 1127.75 1882.41 1.67 0.67
METUNv1_700262 METUNv2_520265 FdhR, Formate dehydrogenase regulator 210.25 231.00 1.10 0.90
METUNv1_570005 METUNv2_400021 FdsA, NAD-dependent, tungsten-containing formate dehydrogenase alpha subunit 1438.46 1527.51 1.06 0.91
METUNv1_570006 METUNv2_400022 FdsB, NAD-dependent, tungsten-containing formate dehydrogenase beta subunit 951.26 1018.18 1.07 0.90
H4F-pathway/Serine cycle/Glyoxylate shunt
METUNv1_460318 METUNv2_290319 FtfL, formate-tetrahydrofolate ligase/synthetase 4729.66 1674.32 −2.82 0.54
METUNv1_460309 METUNv2_290310 Fch, Methenyltetrahydrofolate cyclohydrolase 2091.39 1541.64 −1.36 0.90
METUNv1_460310 METUNv2_290311 MdtA, NADP-dependent methylenetetrahydrofolate dehydrogenase 4363.44 2973.78 −1.47 0.93
METUNv1_460311 METUNv2_290312 Hpr, Hydroxypyruvate reductase, NAD(P)H-dependent. 3620.17 3220.89 −1.12 0.89
METUNv1_460312 METUNv2_290313 Sga, Serine-glyoxylate aminotransferase 10,590.93 8570.41 −1.24 0.79
METUNv1_460313 METUNv2_290314 GlyA, Serine hydroxymethyltransferase 6313.10 5292.54 −1.19 0.87
METUNv1_460314 METUNv2_290315 MtkA, Malate thiokinase large subunit 4955.01 6292.07 1.27 0.73
METUNv1_460315 METUNv2_290316 MtkB, Malate thiokinase small subunit 5081.45 5363.84 1.06 0.73
METUNv1_460316 METUNv2_290317 Ppc1, phosphoenolpyruvate carboxylase 2661.63 2768.19 1.04 0.86
METUNv1_460317 METUNv2_290318 Mcl, malyl-CoA lyase 3260.85 4275.94 1.31 0.58
METUNv1_770329 METUNv2_590331 Gk, Glycerate kinase 1197.01 1116.38 −1.07 0.93
METUNv1_770169 METUNv2_590170 Ppc2, Phosphoenolpyruvate carboxylase 1034.15 1074.00 1.04 0.91
METUNv1_460302 METUNv2_290303 Eno, Enolase 2095.13 1936.51 −1.08 0.92
METUNv1_710053 METUNv2_530053 Pgm, Phosphoglyceromutase 470.57 517.14 1.10 0.90
METUNv1_620020 METUNv2_450021 Ms, Malate synthase A 353.94 269.76 −1.31 0.89
METUNv1_620018 METUNv2_450018 Icl, Isocitrate lyase 12,174.92 8389.98 −1.45 0.85
CO2 Fixation (CBB cycle)
METUNv1_750044 METUNv2_570044 CbbR, RuBisCO operon transcriptional regulator 195.62 189.12 −1.03 0.93
METUNv1_750045 METUNv2_570045 CbbL, Ribulose-1,5-bisphosphate carboxylase large subunit 127.63 130.88 1.03 0.94
METUNv1_750046 METUNv2_570046 CbbS, Ribulose bisphosphate carboxylase small subunit 41.62 47.50 1.14 0.92
METUNv1_750047 METUNv2_570047 CbxX, chromosomal AAA type ATPase 8.75 11.94 1.36 0.89
METUNv1_750048 METUNv2_570048 CbbY, haloacid dehalogenase 7.25 14.56 2.01 0.73
METUNv1_750049 METUNv2_570049 CbbE, Ribulose-phosphate 3-epimerase 13.88 16.13 1.16 0.91
METUNv1_750050 METUNv2_570050 Pgp, phosphoglycolate phosphatase 12.50 13.00 1.04 0.94
METUNv1_750051 METUNv2_570051 CbbF, Fructose-1,6-bisphosphatase 26.75 30.51 1.14 0.92
METUNv1_750052 METUNv2_570052 CbbP, Phosphoribulokinase 26.69 32.00 1.20 0.92
METUNv1_750053 METUNv2_570053 CbbT, Transketolase 24.88 42.56 1.71 0.73
METUNv1_750054 METUNv2_570054 CbbG, Glyceraldehyde-3-phosphate dehydrogenase 17.50 19.56 1.12 0.93
METUNv1_750055 METUNv2_570055 CbbA, Fructose-bisphosphate aldolase 72.75 65.50 −1.11 0.93
METUNv1_700111 METUNv2_520114 CbbR, RuBisCO operon transcriptional regulator 360.50 255.84 −1.41 0.90
METUNv1_760018 METUNv2_580018 CbbQ, Post-translational RubisCO activator 16.25 19.38 1.19 0.91
Sugar Phosphate Interconversions
METUNv1_470279 METUNv2_300282 Fbp, Fructose-1,6-bisphosphatase 403.90 440.81 1.09 0.91
METUNv1_700104 METUNv2_520106 Fba, Fructose-bisphosphate aldolase 920.68 1094.83 1.19 0.85
METUNv1_700105 METUNv2_520107 Pyk, Pyruvate kinase II 807.00 786.28 −1.03 0.92
METUNv1_700106 METUNv2_520108 Pgk, Phosphoglycerate kinase 693.63 684.62 −1.01 0.92
METUNv1_700107 METUNv2_520109 Gapdh, Glyceraldehyde 3-phosphate dehydrogenase 1478.94 1536.51 1.04 0.90
METUNv1_700108 METUNv2_520110 Tk, Transketolase 982.15 730.45 −1.34 0.89
METUNv1_700109 METUNv2_520111 Prk, Phosphoribulokinase 380.94 483.87 1.27 0.82
METUNv1_700110 METUNv2_520112 Fbp3, Fructose-1,6-bisphosphatase 902.64 846.62 −1.07 0.94
METUNv1_470090 METUNv2_300091 Pps, Phosphoenolpyruvate synthase 1610.66 1360.78 −1.18 0.94
METUNv1_460093 METUNv2_290089 Pck, Phosphoenolpyruvate carboxykinase (GTP) 242.99 191.39 −1.27 0.89
METUNv1_580038 METUNv2_410037 Pfk, Pyrophosphate-dependent phosphofructokinase 866.70 1075.27 1.24 0.85
METUNv1_580036 METUNv2_410036 Pyrophosphate-energized inorganic pyrophosphatase 747.52 887.26 1.19 0.88
METUNv1_580049 METUNv2_410048 Pyrophosphate phosphohydrolase 555.12 602.37 1.09 0.89
TCA cycle
METUNv1_700127 METUNv2_520130 E1 component of pyruvate dehydrogenase 959.88 1210.51 1.26 0.80
METUNv1_700126 METUNv2_520129 E2 component of pyruvate dehydrogenase 217.00 302.25 1.39 0.77
METUNv1_700125 METUNv2_520128 E3 component of pyruvate dehydrogenase 482.26 555.08 1.15 0.88
METUNv1_700186 METUNv2_520190 Succinyl-CoA synthetase beta subunit 705.14 556.31 −1.27 0.91
METUNv1_700187 METUNv2_520191 Succinyl-CoA synthetase alpha subunit 622.44 414.43 −1.50 0.86
METUNv1_470127 METUNv2_300128 Fumarate hydratase class I 659.82 590.00 −1.12 0.93
METUNv1_460167 METUNv2_290164 Fumarate hydratase class II (fumarase C) 185.50 141.26 −1.31 0.91
METUNv1_460003 METUNv2_290002 ME1, Malic Enzyme 952.12 789.15 −1.21 0.93
METUNv1_520009 METUNv2_350011 Mdh, Malate dehydrogenase 2673.56 1934.79 −1.38 0.91
METUNv1_520011 METUNv2_350013 Succinate dehydrogenase cytochrome b556 subunit 324.52 191.00 −1.70 0.70
METUNv1_520012 METUNv2_350014 Succinate dehydrogenase anchor subunit 572.13 412.89 −1.39 0.88
METUNv1_520013 METUNv2_350015 Succinate dehydrogenase flavoprotein subunit 4086.04 2492.77 −1.64 0.84
METUNv1_520014 METUNv2_350016 Succinate dehydrogenase Fe–S protein 843.00 709.16 −1.19 0.93
METUNv1_520016 METUNv2_350018 Citrate synthase 2249.29 2218.66 −1.01 0.91
METUNv1_520017 METUNv2_350019 E1 component of alpha-ketoglutarate dehydrogenase 1429.24 1533.03 1.07 0.89
METUNv1_520018 METUNv2_350020 E2 component of alpha-ketoglutarate dehydrogenase 697.75 762.99 1.09 0.90
METUNv1_520019 METUNv2_350021 E3 component of alpha-ketoglutarate dehydrogenase 1364.02 1401.04 1.03 0.89
METUNv1_620012 METUNv2_450011 Isocitrate dehydrogenase kinase/phosphatase 241.63 308.13 1.28 0.84
METUNv1_620013 METUNv2_450012 Isocitrate dehydrogenase (NADP+) 2578.02 2899.29 1.12 0.80
Amino Acid Synthesis
METUNv1_660052 METUNv2_480054 Glutamate synthase (NADPH) large chain (NADPH-GOGAT) 3985.94 2866.80 −1.39 0.87
METUNv1_660053 METUNv2_480055 Glutamate synthase (NADPH) small chain (NADPH-GOGAT) 1357.89 903.01 −1.50 0.91
METUNv1_450044 METUNv2_280044 Glutamine synthetase (Glutamate-ammonia ligase) 2361.78 2288.13 −1.03 0.56
METUNv1_470103 METUNv2_300104 Glutamate dehydrogenase, NADP-specific (NADP-GDH) 1040.15 1519.76 1.46 0.93
METUNv1_470104 METUNv2_300105 Aspartate aminotransferase (Transaminase A) (AspAT) 1020.04 941.84 −1.08 0.92
METUNv1_470184 METUNv2_300183 Putative aspartate transaminase 617.88 620.11 1.00 0.93
METUNv1_750043 METUNv2_570043 Serine-pyruvate aminotransferase 679.01 606.13 −1.12 0.91
Oxidative Phosphorylation
METUNv1_750182 METUNv2_570182 ATP synthase F0, A chain 886.37 965.57 1.09 0.83
METUNv1_750183 METUNv2_570183 ATP synthase F0, C chain 1218.52 1365.89 1.12 0.93
METUNv1_750184 METUNv2_570184 ATP synthase F0, B chain 2528.92 2775.88 1.10 0.89
METUNv1_750185 METUNv2_570185 ATP synthase delta chain 2885.17 2564.77 −1.12 0.86
METUNv1_750186 METUNv2_570186 ATP synthase subunit alpha subunit 8915.44 6780.46 −1.31 0.89
METUNv1_750187 METUNv2_570187 ATP synthase gamma subunit 5294.58 4705.58 −1.13 0.93
METUNv1_750188 METUNv2_570188 ATP synthase beta subunit 9593.01 6768.24 −1.42 0.92
METUNv1_750189 METUNv2_570189 ATP synthase epsilon subunit 1784.40 1464.79 −1.22 0.90
METUNv1_770336 METUNv2_590337 NAD(P) transhydrogenase subunit alpha 858.91 858.01 −1.00 0.93
METUNv1_770337 METUNv2_590338 NAD(P) transhydrogenase subunit beta 399.38 455.25 1.14 0.92
METUNv1_460126 METUNv2_290122 NADH-quinone oxidoreductase chain A 156.74 148.38 −1.06 0.93
METUNv1_460127 METUNv2_290123 NADH-quinone oxidoreductase subunit B 451.50 376.26 −1.20 0.94
METUNv1_460128 METUNv2_290124 NADH (or F420H2) dehydrogenase subunit C 388.51 353.76 −1.10 0.94
METUNv1_460129 METUNv2_290125 NADH-ubiquinone oxidoreductase D subunit 754.27 681.62 −1.11 0.92
METUNv1_460130 METUNv2_290126 NADH-quinone oxidoreductase subunit E 207.95 211.51 1.02 0.92
METUNv1_460131 METUNv2_290127 NADH-quinone oxidoreductase subunit F 628.76 528.76 −1.19 0.87
METUNv1_460132 METUNv2_290128 NADH-quinone oxidoreductase subunit G 1248.13 938.27 −1.33 0.93
METUNv1_460133 METUNv2_290129 NADH-quinone oxidoreductase subunit H 520.13 381.75 −1.36 0.93
METUNv1_460134 METUNv2_290130 NADH-quinone oxidoreductase subunit I 327.57 337.82 1.03 0.94
METUNv1_460135 METUNv2_290131 NADH-quinone oxidoreductase subunit J 131.50 118.13 −1.11 0.88
METUNv1_460136 METUNv2_290132 NADH-quinone oxidoreductase subunit K 58.88 60.63 1.03 0.91
METUNv1_460137 METUNv2_290133 NADH-quinone oxidoreductase subunit L 637.25 465.50 −1.37 0.83
METUNv1_460138 METUNv2_290134 NADH-quinone oxidoreductase subunit M 372.75 302.75 −1.23 0.77
METUNv1_460139 METUNv2_290135 NADH-ubiquinone oxidoreductase, chain N 523.88 339.07 −1.55 0.64
METUNv1_660132 METUNv2_480138 Ferredoxin-NADP reductase 522.14 879.52 1.68 0.69
METUNv1_590030 METUNv2_420029 Ubiquinol-cytochrome c reductase complex, cytochrome c1 2503.55 1092.90 −2.29 0.58
METUNv1_590031 METUNv2_420030 Ubiquinol-cytochrome c reductase complex, cytochrome b 2945.38 1365.76 −2.16 0.81
METUNv1_590032 METUNv2_420032 Ubiquinol-cytochrome c reductase iron-sulfur subunit (Rieske iron-sulfur protein) (RISP) 3125.54 1240.80 −2.52 0.84
METUNv1_590008 METUNv2_420008 Peroxidase/catalase (Catalase-peroxidase) 3075.82 1883.61 −1.63 0.84
METUNv1_670031 METUNv2_490032 Cytochrome c oxidase subunit 1 5577.29 4801.32 −1.16 0.93
METUNv1_670030 METUNv2_490031 Cytochrome c oxidase subunit 2 4105.55 3835.175 −1.07 0.87
METUNv1_670034 METUNv2_490035 Cytochrome c oxidase subunit 3 2746.54 2227.3 −1.23 0.89
METUNv1_670033 METUNv2_490034 Cytochrome c oxidase assembly protein 1365.325 1525.035 1.12 0.93
METUNv1_580051 METUNv2_410050 Hemin uptake protein hemP (fragment) 1319.89 1517.79 1.15 0.68
METUNv1_580052 METUNv2_410051 Bacterioferritin-associated ferredoxin 930.37 739.50 −1.26 0.80
METUNv1_580053 METUNv2_410052 Bfr, Bacterioferritin 1845.02 2568.53 1.39 0.77

* Each sample represents the average of two biological replicates. Values represent reads per kilobase of coding sequence per million (reads) mapped (RPKM).