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. 2015 Sep 18;3(3):567–587. doi: 10.3390/microorganisms3030567

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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The amyA locus, promoter fusion. Top, PBL2025 (wild type), amyA (SSO1172) and flanking ORFs. Bottom, PBL2058 (malAp::amyA) promoter substitution using malAp. DNA sequence of fusion junctions for SSO1171::malAp and malAp::amyA are shown. The malAp promoter elements (BRE, TATA) are boxed, malAp transcription start is indicated (+1), amyA start codon is shown in bold.