(A) Dot plots indicating the overall gating strategy to define the myeloid sub-populations in EG7 tumor mass.
(B) Quantification of tumor-infiltrating myeloid subpopulations in the CD11b+ gate in mice treated (CD8) or untreated (w/o CD8) with ACT, n=18, pooled from 3 experiments
(C, D) Frequency (C, n=18 pooled from 3 experiments) and representative flow cytometry plots (D, percentages of different populations are indicated in each quadrant.) for tumor infiltrating myeloid populations in CD11b+NOS2+ gate (red boxes) in WT mice untreated (w/o CD8) or treated with (CD8) ACT.
(E) Frequency of different myeloid subpopulations in tumor cell suspensions stimulated or not with LPS in CD11b+ gate, n=6 representative experiment of 3.
(F) Sorted myeloid subpopulations were cultured with naive CD4+ lymphocytes to evaluate IFN-γ released in supernatants by ELISA, representative experiment of 3.
(B, C, E, F) Mean ± s.d., ***p ≤ 0.001, **p ≤ 0.01, by using One Way ANOVA.
See also figure S2.