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. Author manuscript; available in PMC: 2017 Sep 12.
Published in final edited form as: Cancer Cell. 2016 Sep 12;30(3):377–390. doi: 10.1016/j.ccell.2016.08.004

Figure 2. Adoptive transfer of tumor-specific CD8+ T cells induces Tip-DC expansion.

Figure 2

(A) Dot plots indicating the overall gating strategy to define the myeloid sub-populations in EG7 tumor mass.

(B) Quantification of tumor-infiltrating myeloid subpopulations in the CD11b+ gate in mice treated (CD8) or untreated (w/o CD8) with ACT, n=18, pooled from 3 experiments

(C, D) Frequency (C, n=18 pooled from 3 experiments) and representative flow cytometry plots (D, percentages of different populations are indicated in each quadrant.) for tumor infiltrating myeloid populations in CD11b+NOS2+ gate (red boxes) in WT mice untreated (w/o CD8) or treated with (CD8) ACT.

(E) Frequency of different myeloid subpopulations in tumor cell suspensions stimulated or not with LPS in CD11b+ gate, n=6 representative experiment of 3.

(F) Sorted myeloid subpopulations were cultured with naive CD4+ lymphocytes to evaluate IFN-γ released in supernatants by ELISA, representative experiment of 3.

(B, C, E, F) Mean ± s.d., ***p ≤ 0.001, **p ≤ 0.01, by using One Way ANOVA.

See also figure S2.