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. 2016 Jun 28;24(8):1378–1387. doi: 10.1038/mt.2016.97

Figure 4.

Figure 4

The integration of the polyA signals upstream of DMPK CTG repeats in DM-03 induced pluripotent stem (iPS) cells led to elimination of nuclei RNA foci in linear-differentiated neural cells. (Upper panel): nuclear RNA foci were only detected in neural stem cells (NSCs) derived from parental DM-03 iPS cells while no foci were detected in normal NSCs or NSCs derived from genome-treated iPS cells. Green: nestin. (Lower panel) nuclear RNA foci were only detected in spontaneous differentiated neurons derived from parental DM-03 iPS cells while no foci were detected in normal neurons or neurons derived from genome-treated iPS cells. Green: neurofilament H. Normal: neural cells from normal iPS cells. DM-03: neural cells from parental DM1 iPS cells. 13-3 and 33-4: neural cells from genome-treated iPS cells with polyA signals (PASs) integrated in DMPK intron 9.