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. 2016 Aug 9;5(8):e344. doi: 10.1038/mtna.2016.50

Figure 2.

Figure 2

DHA-hsiRNA shows slow neuronal uptake, equivalent Huntingtin (Htt) mRNA silencing, and reduced hydrophobicity compared with Chol-hsiRNA. (a) Primary cortical neurons were incubated with Cy3-DHA-hsiRNAHTT and Cy3-Chol-hsiRNAHTT (0.5 µmol/l) for 0–72 hours. Images obtained with Leica inverted microscope (63×). Nuclei (Hoechst), blue; hsiRNA (Cy3), red. (b) Primary cortical neurons were incubated with Cy3-DHA-hsiRNAHTT and Chol-hsiRNAHTT at concentrations shown for 1 week. Level of huntingtin mRNA was measured using QuantiGene (Affymetrix) normalized to housekeeping gene, Ppib (cyclophillin B), and presented as percent of untreated control (n = 3, mean ± SD). UNT, untreated cells. (c) High-performance liquid chromatography (HPLC) traces of DHA-hsiRNAHTT and Chol-hsiRNAHTT following reverse phase chromatography (C8 column). Htt, Huntingtin; DHA, docosahexaenoic acid; DHA-hsiRNA, docosahexaenoic acid-hsiRNA conjugate; Chol-hsiRNA, cholesterol-hsiRNA conjugate.