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. 2016 Aug 2;5(8):e341. doi: 10.1038/mtna.2016.51

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

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Intracellular interaction of FITC-labeled PEA formulated with Cy3-labeled-oligonucleotide (Cy3-Oligo) in C2C12 cell line (5 µg polymer in 500 µl 10% FBS-DMEM with Cy3-Oligo 1 µg). (A1-A5) Red fluorescence from Cy3-Oligo; (B1-B5) Green fluorescence arising from FITC-polymer; (C1-C5) Merged image. The yellow fluorescence indicates that FITC-polymer colocalized with Cy3-Oligo. The images were obtained using a Zeiss LSM-710 inverted confocal microscope with 63× magnification.