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. 2016 Aug 23;5(8):e354. doi: 10.1038/mtna.2016.62

Figure 1.

Figure 1

Regulation of predicted targets by miR-23-27-24 cluster. (a) Predicated and validated targets of miR-23~miR-27~miR-24 clusters known to be present in tight and adherens junctions in ECs. miR-23 includes miR-23a and miR-23b. miR-27 includes miR-27a and miR-27b. Validated targets are in bold. (b) Relative mRNA expression of the predicted miR-23 targets JAM-C, VE-PTP, CCM2, ZO-1 and ZO-2 in HUVEC transfected with miR-23a or miR-23b mimic, LNA to miR-23 or controls. Expression levels were measured by qRT-PCR with results of mRNA normalized to β-actin. mean ± SEM; n = 3. *P < 0.05; **P < 0.005. LNA, locked nucleic acid; HUVEC, human umbilical vein endothelial cells; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction.