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. 2016 Aug 23;5(8):e354. doi: 10.1038/mtna.2016.62

Figure 3.

Figure 3

Mir-23b but not and miR-23a regulates EC tube formation. (a) LNA-23 increased HUVEC tubule formation on Matrigel. (b) Over-expression of miR-23b but not miR-23a inhibits tubule formation on Matrigel. Cells were monitored and photos taken every hour, for 7 hours. Representative images all taken at the same time point for each experiment are shown with a scale bar = 100 μm. Images analysis and quantification were performed by Wimasis software (Wimasis GmbH). Graphs of tube length, number and branching points show the mean ± SEM. *P < 0.05; **P < 0.005; ****P < 0.0001 by unpaired two-tailed t-test. n = 3 independent EC lines. (c) Angiogenic effects of miR-23a and miR-23b in mice. Mice received subcutaneous injection of 0.5 ml of a Matrigel mixture containing FGF-2, Heparin, and mimics. The animals were killed and gels dissected 2 weeks later. 5 μm sections were stained for CD31 (n = 3–4 for each plug) and a representative image is shown. Pixel histogram quantitation of percentages of vessel area (CD31 positive staining) and average vessel size with miR-23a, miR-23b or control mimic is shown. Each group contained 3–4 animals. Scale bar = 200 μm, *P < 0.05, **P < 0.01. NS = not significant. LNA, locked nucleic acid; HUVEC, human umbilical vein endothelial cells.