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. 2016 Aug 23;5(8):e354. doi: 10.1038/mtna.2016.62

Figure 6.

Figure 6

Physiological regulation of miR-23 paralogues. (a) Expression of miRNA-23a and miR-23b in 3–4 days postseeding. Data are normalized to levels on Day 1 after seeding, which did not differ from Day 0. (b) Expression of miR-23a, miR-23b, and mRNA for JAM-C and ZO-2 24 hours after seeding at different density. Density 1, 2, and 3 corresponds to 1 × 105, 2 × 105, and 3 × 105 HUVECs per well of six-well plate. Expression levels were measured by qRT-PCR, with the results of microRNA normalized to U48 and mRNA normalized to β-actin. Graph represents mean ± SEM; n = 5 independent experiments. *P < 0.05, **P < 0.01. (c) Schematic of proposed function of miR23a and miR-23b in the regulation of EC function. LNA, locked nucleic acid; HUVEC, human umbilical vein endothelial cells; PCR, polymerase chain reaction.