Fig 3.

Qualitative HPLC analyses of ergot alkaloids from wild type, easM knockout (easM ko), and easM complemented (easM co) strains of N. fumigata. Data were collected with a fluorescence detector with excitation and emission settings of 272 nm and 372 nm, respectively. Peaks corresponding to ergot alkaloids are labeled only in the wild-type chromatogram. The peak with a m/z value 309.3 in LC/MS analyses, consistent with the [M+H]⁺ of a prenylated form of festuclavine, is labeled. The insert in each chromatogram shows a close up of time range in which fumigaclavine B and chanoclavine-I eluted