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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Curr Genet. 2016 Mar 14;62(4):853–860. doi: 10.1007/s00294-016-0591-5

Table 1.

PCR primers and products

Primer combination Primer sequence (5′ to 3′) Product Product length in wild type Product length in knockout
easMF + easMR GCCATTCCTCCACTCTTCAG + CCAAAGAACATTCCCAGCTC easM 3067 bp 3416 bp
M5F + HY ACTGCGCTACAGTCGATGTAAC + GGATGCCTCCGCTCGAAGTA 5′ flank, ko locus No product 2916 bp
M3F + YG GACTGCCAGTATCATCTACC + CGTTGCAAGACCTGCCTGAA 3′ flank, ko locus No product 2057 bp
SmaHygF + ClaHygR CATGCCCGGGGGCTTGGCTGGAGCTAGTGG + CTGTATCGATCCGTGGAGGTAATAATTGACG hph1 No product 1441 bp