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. Author manuscript; available in PMC: 2017 Sep 25.
Published in final edited form as: J Mol Biol. 2016 Mar 24;428(19):3776–3788. doi: 10.1016/j.jmb.2016.03.017

Fig. 6. Signaling properties of TsrΔI214 derivatives in an adaptation-deficient host.

Fig. 6

Mutant derivatives of plasmid pPA114 were examined by FRET kinase assay in strain UU2567 (CheR− CheB−). The dashed line shows the Hill fit (with data points omitted) for the dose-response of wild-type Tsr. Solid lines (with data points) are Hill fits for the indicated Tsr-ΔI214 derivatives. K1/2 and Hill coefficient values for these serine-responsive receptors were: 18 μM, 14 (Tsr-wt); 15 μM, 2.9 (ΔI214/K215G); 22 μM, 4.3 (ΔI214 [QQQEE]); 34 μM, 3.8 (ΔI214 [QEQQE]). The parental mutant receptor [ΔI214/(K215)] and two of its derivatives (K215D and K215N) were locked-OFF (horizontal white bar); the K215A derivative was locked-ON (horizontal black bar).