Fig. 1.

Akt1 deficiency compromises endothelial-barrier integrity and modulates VEGF and Ang-1-mediated endothelial-barrier function. a Real-time changes in the barrier resistance of ShControl and ShAkt1 HMEC monolayers as measured using ECIS equipment showing differences in endothelial-barrier resistance between ShControl and ShAkt1 HMEC monolayers at various time points after plating equal amount of cells in array wells (n = 3). b Graph showing quantification of a comparison between ShControl and ShAkt1 HMEC on the acute as well as chronic effects of optimal dose (20 ng/ml) of VEGF on barrier integrity (n = 3). c Bar graph showing quantification of a comparison between control and ShAkt1 knockdown HMEC on the acute as well as chronic effects of optimal dose of Ang-1 (50 ng/ml) on their barrier-junction integrity (n = 3). d Bar graph showing quantification of a comparison between control and ShAkt1 knockdown HMEC on the acute as well as chronic effects of supra-optimal dose of VEGF (50 ng/ml) alone and in combination with Ang-1 (50 ng/ml) on their barrier-junction integrity (n = 3). *P < 0.01