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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Traffic. 2016 Jul 24;17(10):1091–1109. doi: 10.1111/tra.12423

Figure 1. Sec18p preferentially binds to liposomes containing phosphatidic acid.

Figure 1

Recombinant His6-Sec18p (2 µg) was incubated with liposomes of the indicated compositions for 10 min at 30°C. Liposomes were isolated by centrifugation and washed with 1X PBS before bound proteins were resolved by SDS-PAGE. Bar graphs show average normalized densitometry values measured for 3 separate experiments. Binding was observed in liposomes with PA or DAG (A), with increasing concentrations of PA or DAG (B), with increasing concentrations of PA or PS (C), and with no liposomes (D). * P<0.05; ** P<0.001; *** P<0.0001