Six hours after the last ethanol treatment, mice were sacrificed and the pancreas was removed and analyzed for the expression of TNFα, IL-1β, IL-6, MCP-1 and CCR2 by immunoblotting (A and E). The expression of these cytokines and chemokines was quantified and normalized to the expression of α-tubulin (B, C, D, F and G). Each data point was the mean ± SEM of three animals. * denotes statistical difference (p<0.05) and ** denotes significant difference (p<0.01).