Figure 1. Ethidium bromide reduces mtDNA copy number.
Chronic, lifelong exposure to EtBr reduced (A) mtDNA copy number (2 way ANOVA, main effect of time (p<0.0001), but not EtBr (p=0.073), and their interaction (p=0.013)), but not (B) nucDNA copy number (2 way ANOVA, main effect of time (p<0.0001), but not EtBr (p=0.089), or their interaction (p=0.36)), while also reducing the (C) mtDNA:nucDNA ratio (2 way ANOVA, main effect of time (p<0.0001), EtBr (p<0.0001), and their interaction (p=0.0073). Asterisk denotes statistical significance (p<0.05) for post-hoc comparison (Tukey's HSD) to control within each time point. N=6–9. Bars ± SEM.