Figure 8.
HLA-universal megakaryocytes (MKs) produce platelets (PLTs) after transfusion into a mouse model. Mice were transfused with 3 × 106 HLA-universal iPSC-derived MKs by tail vein injection. Twenty minutes before transfusion of MKs, the mice get immunized by injection of anti-HLA-A*02 antibody. Nonimmunized mice served as a control. Frequencies of human PLTs were detected before transfusion as well as 1 and 4 h after MK infusion by flow cytometric analysis of the PB. (A) Representative dot plots displaying human PLTs detected in the mouse circulation. The gate indicates the frequency of human PLTs. (B) The graph shows mean and SD of the PLT frequencies detected after HLA-universal MK transfusion in three independent experiments. **p ≤ 0.01.