Table 5.
The following data were obtained from standard, in duplicate, over the range of 0–5 ng/mL of Cytochrome c
| Concentration of Cytochrome c standard (ng/mL) | OD for standard 1 (nm) | OD for standard 2 (nm) | Average OD (nm) |
|---|---|---|---|
| 5 | 1.864 | 1.9356 | 1.8998 |
| 2.5 | 1.0343 | 0.9942 | 1.01425 |
| 1.25 | 0.5594 | 0.5659 | 0.56265 |
| 0.625 | 0.3851 | 0.3957 | 0.3904 |
| 0.312 | 0.3566 | 0.3195 | 0.33805 |
| 0.156 | 0.2873 | 0.2927 | 0.2900 |
| 0.078 | 0.2810 | 0.2836 | 0.2823 |
| 0 | 0.2568 | 0.2599 | 0.25835 |
A standard curve was developed to determine the protein concentration in the samples