Fig. 5.

Population doubling assay and SA-β-Gal staining of wild type and Lowland Anoa derived recombinant cells. a Population doubling assay in 4 cell lines; WT, EGFP, K4D, and K4DT cell lines. K4DT cells showed continued cell proliferation beyond passage 26, while the wild type and EGFP transfected cells ceased to divide at around PD value of 60. b The result of SA-β-Gal staining in wild type, EGFP expressing cells (EGFP), K4D and K4DT cell lines at passage 24. Note that all of the wild type, EGFP, and K4D cells showed positive blue staining, while the K4DT cells were negative for SA-β-Gal staining, and maintained small cell size