Fig. 4.

Effects of FA on mature osteoclast numbers. a Mature osteoclasts generated from RAW264.7 macrophages were treated with FA for 24 and 48 h respectively and TRAP+ stained osteoclasts were photographed. b TRAP+ stained osteoclasts containing >3 nuclei were counted and represented in a graphical format (scale bars 200 µm). Data are expressed as mean ± SD and are representative of three independent experiments. (***P < 0.001 vs RANKL)