Table 2.

Expression and functionality criteria, respectively, used to select physiological effects reported in literature that were most likely mediated by the P2Y₁₁ receptor

Expression criteria  1. Non-murine species  2. Specific P2RY11/PPAN-P2RY11 PCR primers or WB with 40 ± 10 kDa band

Functionality criteria  1. Use tissue/cells that fulfill the expression criteria  2. At least two of the following:   • Use of NF546/NF157/NF340   • P2RY11 RNA interference   • Negative in test for other P2 receptors including P2Y1 and/or P2X7

Only tissue and cells from a non-murine species using PCR primers specific to P2RY11/PPAN-P2RY11 or detecting protein on western blot (WB) with a size in the expected range of 40 ± 10 kDa were considered to express the P2Y₁₁ receptor. Studies that used tissue or cells that fulfilled the expression criteria and applied at least two of three different approaches to prove the activation of the P2Y₁₁ receptor were considered most likely to describe an effect mediated by the P2Y₁₁ receptor. Many studies have investigated the role of the P2Y₁₁ receptor but have not fulfilled these criteria [11–16, 18, 21, 24–26, 36, 41–43, 55, 56, 61, 67, 74, 82, 83, 86–122]