Fig. 11.

Co-immunoprecipitation of P2X2 and Pirt. Lysates from proximal colon tissue were immunoprecipitated with anti-Pirt or anti-P2X2 antibodies, followed by immunoblotting with anti-P2X2 or anti-Pirt antibodies (line 1 and lane 3), respectively. Immunoprecipitation with control resin for negative control (line 2 and lane 4). Input represents 5 % of the lysates used for the immunoprecipitation reaction. The data are representative of three similar experiments. IP immunoprecipitation, IB immunoblot