Fig. 4.

There are more osteoclasts in bone of A2BRKO mice- Following decalcification of bone and paraffin embedding sections were stained for TRAP+ cells, as described in “Material and methods” and the number of TRAP+ cells/hpf was determined. a Representative images of subchondral femoral bone stained with TRAP are shown on the bottom of the graphs and plotted are the number of osteoclasts per bone surface in subchondral bone of 6- and 12-week-old mice. b Representative images of distal bone and the number of osteoclasts/area of bone is shown. Data are expressed as the mean ± SEM (n = 4 for each group; Student’s t test: *p < 0.05; **p < 0.01)