Fig. 7.

Mature osteoblasts isolated from A2BRKO mice produce more calcified matrix, collagen, and alkaline phosphatase than osteoblasts isolated from WT mice—mature osteoblasts were isolated from the long bones of A2BRKO and WT mice, cultured and then stained for a calcified matrix production (Alizarin Red); b collagen deposition (Sirius Red); and c alkaline phosphatase activity (n = 4 experiments performed in duplicate). Data are expressed as mean ± SEM (Student’s t test: *p < 0.05; **p < 0.01)