Fig. 5.

The role of P2X3 receptors in activation of trigeminal nerve terminals in meninges. a Representative traces of trigeminal nerve spikes in meningeal hemiskull preparation in control and after application of 20 μM α,β-meATP. b The time course of changes in the frequency of meningeal spikes after α,β-meATP application (n = 5 hemiskull preparations); *p < 0.05 by the Student-paired t test. c The time course of changes in the frequency of meningeal spikes after application of 20 μM α,β-meATP in the presence of 10 μM A-317491 (n = 5 hemiskull preparations). Notice that this P2X3 antagonist completely prevented the facilitatory effect of α,β-meATP