Fig. 3.

MyD88 involvement on L. monocytogenes effect on 5-HT uptake. Cells were infected with L. monocytogenes at MOI 100 and 200 for 2 h and/or MyD88 inhibitor (100 μM 1 h previous and during the infection with Listeria) and compared with untreated cells (control). Uptake of 5-HT was measured after 6 min of incubation, and 5-HT concentration was 0.2 μM. The results are expressed as the percentage of the uptake control and are the mean ± SE of three biological replicates in four independent experiments (n = 12). ***P < 0.001 compared with the control. ^### P < 0.001 and ^## P < 0.01 compared with the corresponding MOI effect without MyD88 inhibitor