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. 2016 Aug 3;72(3):730–739. doi: 10.1007/s00248-016-0809-6

Fig. 4.

Fig. 4

TLR2 involvement on L. monocytogenes effects. a Cells were infected with L. monocytogenes at MOI 200 for 2 h and/or TLR2 antibody (1 μg 30 min previous the infection) and compared with untreated cells (control). Uptake of 5-HT was measured after 6 min of incubation, and 5-HT concentration was 0.2 μM. The results are expressed as the percentage of the uptake control and are the mean ± SE of three biological replicates in four independent experiments (n = 12). ***P < 0.001 compared with the control. b Quantitative RT-PCR analysis of TLR2 mRNA expression in cells infected with L. monocytogenes for 2 h at MOI 200. Relative quantification was performed using comparative Ct (2−ΔΔCt) of three biological replicates in five independent experiments (n = 15). Results are expressed as arbitrary units of control = 1. ***P < 0.001 compared with the control value. c Expression and quantification of TLR2 protein levels in cell lysate using β-actin as an internal control of the protein load (TLR2/β-actin ratio). The results are expressed as a percentage of the control value and are the mean ± SEM of two biological replicates in four independent experiments (n = 8). **P < 0.01 compared with the control value