Fig. 5.

TLR10 involvement on L. monocytogenes effects. a Cells were infected with L. monocytogenes at MOI 200 for 2 h and/or TLR10 antibody (1 μg 30 min previous the infection) and compared with untreated cells (control). Uptake of 5-HT was measured after 6 min of incubation, and 5-HT concentration was 0.2 μM. The results are expressed as the percentage of the uptake control and are the mean ± SE of three biological replicates in four independent experiments (n = 12). ***P < 0.001 compared with the control. ^### P < 0.001 compared with MOI 200 effect without antibody. b Quantitative RT-PCR analysis of TLR10 mRNA expression in cells infected with L. monocytogenes for 2 h at MOI 200. Relative quantification was performed using comparative Ct (2^−ΔΔCt) of three biological replicates in five independent experiments (n = 15). Results are expressed as arbitrary units of control = 1. ***P < 0.001 compared with the control value. c Expression and quantification of TLR10 protein in cell lysate using β-actin as an internal control of the protein load (TLR10/β-actin ratio). The results are expressed as a percentage of the control value and are the mean ± SEM of two biological replicates in four independent experiments (n = 8)