Table 2.

H3Pser10 and H3K4me3 staining patterns in Strongyloididae males

Nematode (male)	Phylogenetic distance to S. ratti	Gonadal organization similar to Stongyloides	Nematode clade	Lifestyle/mode of reproduction	Distal arm		Gonadal loop
					H3Pser10	H3K4me3	H3Pser10	H3K4me3
S. ratti	–	–	IV	Free-living gonochorist	Absent	Present (high)	Present high (nuclear localization distinct from H3K4me3)	Present high
S. papillosus	Closest Strongyloides species	Yes	IV	Free-living gonochorist	Absent	Present (high)	Present high (entire nucleus)	Present high
P. trichosuri	Closest outgroup species	Yes	IV	Free-living gonochorist	Absent	Absent	Present (condensed meiotic chromosomes)	Absent