Fig. 3.

CreC genomic insertion site. The structure of the insertion site in line CreC is shown at the nucleotide level. Four sequences are shown, as follows: (1) attB: the sequence of the attB site in construct BN-B-act-Cre-attB. The full attB site is shown. A partial deletion of the attB site occurred in the CreC insertion, as indicated. (2) Chr1: sequence of the region of chicken chromosome 1 where the transgene inserted in CreC. A small deletion of the genome occurred, as indicated. (3) 5′ side of insertion: the sequence of the transgene insertion on the 5′ side (the side closest to the plasmid backbone), showing the genome/transgene junction. (4) 3′ side of insertion: the sequence of the transgene insertion on the 3′ side (the side closest to Cre), showing the genome/transgene junction