Figure 2.

Effects of 6-aminopyrimidine inhibitors on the clonogenic survival of HCT116 and HT29 colorectal cancer cells.(A) HCT116 cells were treated with increasing concentrations of 1 for 8 h. The medium was replaced with normal growth medium and the cells allowed to form colonies for 1 week. Colonies were stained with 0.4% (v/v) crystal violet and counted. (B) HT29 cells were treated as described in (A). (C) HCT116 cells were treated with the GI₅₀ concentration (or 100 μM, Table 2) of the 6-aminopyrimidine inhibitors for 72 h before being re-plated for analysis of colony formation as described in (A). All experiments were performed in triplicate and the data are the mean±s.d.