Table 1.
Epitopes predicted by bioinformatics on E protein with conservancy results.
| ID of the predicted epitope | #Epitope sequence | Epitope length (a.a.) | Method of prediction (score) | Percentage conservancy (min%) | ||||
|---|---|---|---|---|---|---|---|---|
| Intraserotype | Pan-serotype | |||||||
| DENV1 | DENV2 | DENV3 | DENV4 | |||||
| Entire E protein sequence | 96 | 93 | 97 | 95 | 63 | |||
| EP1/E | SRDFVEGLSGATW ∗8–20/DI |
13 | BepiPred (−1.6–0.26) | 84 | 100 | 100 | 92 | 69 |
| EP2/E | SGATWV ∗16–21/DI |
6 | Ellipro (0.5) | 83 | 100 | 100 | 83 | 66 |
| EP3/E | CVTTMAKDKPTL ∗30–41/DI |
12 | BepiPred (−0.06–0.95) | 75 | 92 | 92 | 100 | 75 |
| EP4/E | TEVTNPAV ∗48–55/DI&DII |
8 | BepiPred (−0.13–0.71) | 88 | 88 | 100 | 88 | 13 |
| EP5/E | EAKISNTTTDSRCPTQGEATLVEEQDANFV CRRTFVDRGWGNGCGLFGKGSLITCAKFKCVT ∗62–123/DII |
62 | Ellipro (0.8) | 96 | 93 | 96 | 69 | 64 |
| EP6/E | AKISNTTTDSRCPTQGEATLVEEQDAN ∗63–89/DII |
27 | BepiPred (−0.12–1.6) | 96 | 93 | 93 | 93 | 67 |
| EP7/E | VDRGWGNGCGLF ∗97–108/DII |
12 | BepiPred (−0.05–0.87) | 100 | 83 | 100 | 100 | 83 |
| EP8/E | HTGDQHQVGNESTEHGTTATITPQAPTTEIQLT ∗144–176/DI |
33 | BepiPred (0.12–1.7) | 88 | 85 | 88 | 90 | 42 |
| EP9/E | PQAPTTEIQLTDYGALTL ∗165–182/DI |
17 | Ellipro (0.6) | 88 | 94 | 88 | 94 | 35 |
| EP10/E | ALTLDCSPRTGLD ∗180–192/DI |
13 | BepiPred (−0.05–0.9) | 100 | 85 | 100 | 100 | 54 |
| Ep11/E | LPWTSGASTSQETWNR ∗218–233/DII |
16 | BepiPred (0.06–1.8) | 94 | 88 | 88 | 81 | 37 |
| EP12/E | GASTSQETW ∗223–231/DII |
9 | Ellipro (0.6) | 88 | 77 | 77 | 77 | 44 |
| EP13/E | LVTFKTAHAKKQEVVVLGS ∗237–255/DII |
19 | Ellipro (0.7) | 94 | 94 | 100 | 84 | 63 |
| EP14/E | TAHAKKQ ∗242–248/DII |
7 | BepiPred (−0.04–0.78) | 86 | 86 | 100 | 100 | 43 |
| EP15/E | VLGSQEGAMHTALTGATEIQTSGTTTI ∗252–278/DII & DI |
27 | BepiPred (−0.03–1.66) | 93 | 96 | 96 | 89 | 55 |
| EP16/E | FAGHLKCRLKMDKLTLKGMS ∗279–298/DI |
20 | SVMTriP20 (0.9) | 90 | 100 | 85 | 95 | 65 |
| EP17/E | LKMDKLTLKGMSYVMCTGSFKLEKEVA ∗287–313/DI&DIII |
27 | Ellipro (0.6) | 92 | 88 | 92 | 96 | 48 |
| EP18/E | FKLEKEVAETQHGT ∗306–319/DIII |
14 | BepiPred (−0.14–0.95) | 100 | 71 | 100 | 93 | 50 |
| ¥EP19/E | KEVAETQHGTVLVQIKYEGT ∗310–329/DIII |
20 | SVMTriP20 (1.0) | 90 | 80 | 95 | 85 | 55 |
| EP20/E | VQIKYEGTDAPCKIPFSTQDEKGVTQNG ∗322–349/DIII |
28 | Ellipro (0.7) | 92 | 82 | 96 | 85 | 35 |
| EP21/E | YEGTDAPCKIPFSTQDEKGVTQNGRLIT ∗326–353/DII |
28 | BepiPred (−0.16–1.5) | 93 | 86 | 96 | 82 | 36 |
| EP22/E | PIVTDKEKPVNIEAEPPFGES ∗356–376/DIII |
21 | BepiPred (−0.12–1.7) | 86 | 86 | 90 | 90 | 43 |
| EP23/E | PIVTDKEKPV ∗356–365/DIII |
10 | Ellipro (0.6) | 80 | 80 | 80 | 80 | 10 |
| ¥EP24/E | PPFGESYIVIGAGEKALKLSWFKKGSSIGKMF ∗371–402/DIII & Stem |
32 | Ellipro (0.6) | 93 | 81 | 93 | 93 | 59 |
| EP25/E | IGAGEKA ∗380–386/DIII |
7 | BepiPred (−0.03–0.2) | 71 | 86 | 71 | 71 | 29 |
| EP26/E | KKGSSIGKMFEATARGA ∗393–409/C-terminus |
17 | BepiPred (−0.04–0.6) | 94 | 88 | 94 | 88 | 71 |
| EP27/E | GDTAWDFGSIGGVFTSVGKL ∗416–435/C-terminus |
20 | SVMTriP20 (0.9) | 95 | 95 | 95 | 95 | 75 |
| EP28/E | DTAWDFGSIGGVFT ∗417–430/C-terminus |
14 | BepiPred (−0.06–0.6) | 86 | 92 | 100 | 92 | 71 |
| EP29/E | IGGVFTSVGKLVHQIFGTAYG ∗425–445/C-terminus |
21 | Ellipro (0.6) | 85 | 95 | 91 | 95 | 55 |
| EP30/E | TMKIGIGVLLTWLGLNSRSTSLSMTCIAVGLITLY ∗454–488/C-terminus |
35 | Ellipro (0.8) | 91 | 91 | 94 | 88 | 42 |
| EP31/E | IGVLLTWLGLNSRSTSLSM ∗459–478/C-terminus |
19 | SVMTriP20 (0.8) | 89 | 94 | 94 | 94 | 52 |
| EP32/E | NSRSTSL ∗469–475/C-terminus |
7 | BepiPred (−0.14–0.2) | 86 | 100 | 86 | 100 | 57 |
∗Sequence position/domain. #Epitope sequence is given with reference to DENV1 isolate AY713476. ¥Epitopes that have been previously described by other authors (cited in the Discussion).