Table 2.
Epitopes predicted by bioinformatics on prM protein with conservancy results.
| ID of the predicted epitope | #Epitope sequence | Epitope length (a.a.) | Method of prediction (score) | Percentage conservancy (min%) | ||||
|---|---|---|---|---|---|---|---|---|
| Intraserotype | Pan-serotype | |||||||
| DENV1 | DENV2 | DENV3 | DENV4 | |||||
| Entire prM protein sequence | 93 | 87 | 98 | 96 | 63 | |||
| EP1/prM | FHLTTRGGE ∗1–9 |
9 | Ellipro (0.6) | 88 | 77 | 88 | 88 | 55 |
| ¥EP2/prM | TTRGGEPHMIVSKQERG ∗4–20 |
17 | BepiPred (0.05–1.1) | 88 | 71 | 94 | 94 | 59 |
| ¥EP3/prM | SKQERGKS ∗15–22 |
8 | Ellipro (0.5) | 87 | 37 | 87 | 87 | 25 |
| EP4/prM | KTAEG ∗26–30 |
5 | Ellipro (0.5) | 80 | 60 | 100 | 100 | 60 |
| EP5/prM | IAMDL ∗37–41 |
5 | Ellipro (0.5) | 100 | 40 | 100 | 80 | 40 |
| EP6/prM | LCEDTMTYKCPRITEAEPDDVDCWCNATDTWVTYGTCSQTGEHRRDKRSV ∗44–93 |
50 | BepiPred (−0.02–1.7) | 82 | 92 | 96 | 96 | 68 |
| ¥EP7/prM | RITEAEPDDVD ∗55–65 |
11 | Ellipro (0.6) | 36 | 81 | 81 | 90 | 18 |
| EP8/prM | VALAPHVGLGLETRTETWMS ∗93–112 |
20 | SVMTriP20 (1.0) | 95 | 100 | 95 | 90 | 75 |
| EP9/prM | LETRTETWMSSEGAWKQIQKV ∗103–123 |
21 | BepiPred (−0.04–0.8) | 90 | 95 | 95 | 95 | 57 |
| EP10/prM | TWALR ∗125–129 |
5 | BepiPred (−0.09–0.1) | 80 | 80 | 80 | 100 | 60 |
| EP11/prM | ALRHPGFTIALFLAHAIGT ∗127–146 |
20 | SVMTriP20 (0.8) | 90 | 95 | 85 | 90 | 50 |
| EP12/prM | GAWKQIQRVETWALRHPGFTVILALFLAH AIGTSITQKGIIFILLMLVTPS ∗115–165 |
50 | Ellipro (0.7) | 86 | 80 | 94 | 94 | 46 |
| EP13/prM | GTSITQ ∗145–150 |
6 | BepiPred (−0.06–0.4) | 100 | 83 | 83 | 100 | 33 |
∗Sequence position. #Epitope sequence is given with reference to DENV1 isolate AY713476. ¥Epitopes that have been previously described by other authors (cited in the Discussion).