Figure 4.

Mitotic co-ordination of membrane and cytoskeletal remodeling by ESCRT-III. A. Cartoon depicting similarities between ESCRT-III dependent nuclear envelope reformation (a) and cytokinesis (b). Enlargement of green-boxed region given in lower cartoon. In both cases, prior to ESCRT-III-dependent membrane resolution, microtubules must be coordinately removed through the action of the AAA-ATPase Spastin, which is recruited to the site of remodeling through interaction with IST1 during nuclear envelope reformation, or IST1/CHMP1B during cytokinesis. In the case of cytokinesis, abscission occurs at a constriction upon the midbody arms and whilst cuts can occur on both sides of the Flemming body, here a single cut is depicted with the asymmetric resorption of the Flemming body by one daughter cell as a midbody remnant.