Figure 3.

NF-κB1is a target gene of miR-9. (A) Binding of miR-9 to NF-κB1 3′-UTR predicted by Target Scan. (B) Dual luciferase reporter gene assay revealed that miR-9 significantly decreased the luciferase activity of NF-κB1 wild-type (wt) 3′UTR. (C) NF-κB1 mRNA levels in chondrocytes transfected with miR-9 mimics, miR-9 inhibitors, or scramble sequence were examined by qRT-PCR. (D) NF-κB1 protein levels were detected by western blotting using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. The results were from 3 independent experiments. The data are presented as the mean ± SD. ∗∗P < 0.01 versus corresponding control; ∗P < 0.05 versus corresponding control.