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. 2016 Sep 7;7:12538. doi: 10.1038/ncomms12538

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Copyright © 2016, The Author(s)

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(a) Structure of the ruthenium complexes RuL2 and RuL3. (b) Subcellular localization and catalytic activity of RuL2 and (c) RuL3. (A) Mitochondrial labelling with TMRE (red), (B) emission of the metal complex (blue), (C) merging of A and B, (D) Rho fluorescence in cells preincubated with the ruthenium catalysts after addition of Rho-alloc, (E) merging of A and D, and (F) merging of B and D. Reaction conditions: cells were incubated with the catalyst (50 μM) and TMRE (100 nM) for 30 min, followed by two washings with DMEM-FBS and treatment with Rho-alloc (100 μM) for 30 min. Scale bar, 12.5 μm.