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. 2015 Jan 22;35(7):1877–1885. doi: 10.1111/liv.12731

Figure 4.

Figure 4

High Mobility Group Box 1 induces autophagy. (A‐B) HMGB1 induces autophagy in a dose dependent manner. Primary rat HSCs on day 4 were treated with different concentration of HMGB1 (0.03–1 μg/ml) for 24 h (A) as indicated, followed by evaluation of autophagy by using immunoblotting with specific antibodies. Protein ratios of LC3‐II(normalized to GAPDH) were used to quantify fold change relative to control and are shown(B), *, P < 0.05,**, P < 0.01. (C–D) HMGB1 induces autophagy flux. Primary rat HSCs on day 4 were incubated with 1 μg/ml HMGB1 for 20 h, followed by 100 nM bafilomycin‐A1 (BA) for 4 hours. Extracts were subjected to autophagy activity and HSCs activation analysis by using immunoblotting with specific antibodies. Protein ratios of LC3‐II(normalized to GAPDH) were used to quantify fold change relative to control and are shown(B), *, P < 0.05,**, P < 0.01.