Reference peptide |
Chemical synthesis using labeled amino acids |
Heavy isotope labeled amino acids |
Amino acid analysis Frank et al. 100, Arsene et al. 142
|
AQUA (absolute quantification) |
Gerber et al. 63
|
|
Chemical postsynthesis tagging |
Isotope‐labeled tags |
Weighing |
ICPL |
Hochleitner et al. 70
|
Reference concatamer |
Metabolic labeling |
15N label |
Not necessary for stoichiometries |
QconCAT |
Beynon et al. 92
|
|
Enzymatic labeling in vivo |
Heavy isotope labeled amino acids |
Not necessary for stoichiometries |
PCS (peptide concatenated standard) |
Kito et al. 77
|
|
In vitro translation incorporating labeled amino acids |
Heavy isotope labeled amino acids: K |
Not necessary for stoichiometries |
polySIS |
Anderson et al. 93
|
|
Chemical synthesis, trypsination, labeling |
mTRaq |
Equalization using a concatenated reporter peptide |
EtEP (equimolarity through equalizer peptide) |
Holzmann et al. 72
|
Reference protein |
In vitro translation (E. coli extract) incorporating labeled amino acids |
Heavy isotope labeled amino acids: R/K |
Amino acid analysis |
PSAQ (protein standard for absolute quantification) |
Brun et al. 94 Protein synthesis: Torizawa et al. 95
|
|
In vitro translation (wheat germ extract) incorporating labeled amino acids |
Heavy isotope labeled amino acids: R/K |
Quantification of a tag peptide after digestion using the AQUA approach |
Flexiquant |
Singh et al. 96 Protein synthesis: Endo and Sawasaki 97
|
|
In vitro translation (E. coli extract) incorporating labeled amino acids |
Heavy isotope labeled amino acids: R/K+ selenomethionine |
ICPMS quantification of selenomethionine |
RISQ |
Zinn et al. 98
|
|
Metabolic labeling in vivo (auxotrophic E. coli strain) or upon in vitro translation |
Heavy isotope labeled amino acids: R/K |
Amino acid analysis |
Absolute SILAC |
Hanke et al. 99
|