Table 1.
Characteristics of bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| JM109 | Plasmid propagation strain | Promega |
| XL10‐Gold | Expression strain for pQE70‐based plasmids | Stratagene |
| BL21‐Gold (DE3) | Expression strain for pCold TF‐based plasmids | Stratagene |
| N. meningitidis | ||
| MC58 | Wild‐type serogroup B strain | ATCC |
| MC58Δapp | app replaced with Ω cassette | Hadi et al. (2001) |
| MC58ΔmspA | mspA replaced with kanamycin cassette | This study |
| MC58ΔappΔmspA | mspA mutant derivative of MC58Δapp | This study |
| Plasmids | ||
| pTOPOMspAflΔΩ | mspA mutagenesis plasmid containing Ω cassette | Turner et al. (2006) |
| pJMK30 | Source of kanamycin resistance cassette | van Vliet et al. (1998) |
| pTOPOMspAflΔKan | mspA mutagenesis plasmid containing kanamycin cassette | This study |
| pGEM‐T Easy | PCR cloning vector | Promega |
| pGEMTEpdapp | Region of MC58 app encoding 43G–1179N in pGEM‐T Easy | This study |
| pGEMTEpdmspA | Region of MC58 mspA encoding 27S–1152A in pGEM‐T Easy | This study |
| pQE70 | Expression vector | Qiagen |
| pQE70pdapp | Expression vector encoding 6 × histidine‐tagged 43G–1179N fragment of MC58 App | This study |
| pQE70pdmspA | Expression vector encoding 6 × histidine‐tagged 27S–1152A fragment of MC58 MspA | This study |
| pCold TF | Protein expression vector | Takara Bio |
| pCold TF‐App | Expression vector encoding 6 × histidine‐tagged 43G–1179N of MC58 App fused to trigger factor | GenScript |
| pCold TF‐MspA | Expression vector encoding 6 × histidine‐tagged 27S–1152A of MC58 MspA fused to trigger factor | This study |