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Primers used in this study
| Primer | Sequence | Restriction site |
|---|---|---|
| app‐QE70‐For_nos | CGCGCATGCATGGACACACTTATTTCGGCATC | SphI |
| app‐QE70‐Rev_noB | GCGAGATCTATTGGCATAACGGCTGATC | BglII |
| mspa‐QE70‐For_nos | CGCGCATGCAATCCATTGTCCGCAACGATG | SphI |
| mspa‐QE70‐Rev_noB | GCGAGATCTGGCCGACCGGCTGAT | BglII |
| pColdTF‐MspA(F) | GGCTGGTACCATGCATTCCATTGTCCGCAAC | KpnI |
| pColdTF‐MspA(R) | GCGTAAGCTTTTATTAAGATCTGGCCGACCGGCTG | HindIII |
