Figure 2. BG-4 purified from Momordica charantia caused a dose-dependent cytotoxicity to HCT-116 and HT-29 human colon cancer cells.

(a) BG-4 calculated ED₅₀ values for 24 and 48 h treatments were 251.3 and 134.4 μg/mL for HCT-116, respectively and (b) BG-4 calculated ED₅₀ values for 24 and 48 h treatments were 249.2 and 217.0 μg/mL for HT-29, respectively. Representative phase-contrast images of human colon cancer cells as affected by BG-4 treatment after 48 h showed changes in the morphology of human colon cancer cells consistent with its cytotoxic activity. Scale bar is 200 μm. Mean values within cell line represented as bars with different letter(s) are statistically different from each other (P < 0.05, n > 3).