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. Author manuscript; available in PMC: 2017 Jun 6.
Published in final edited form as: Curr Biol. 2016 May 19;26(11):1404–1415. doi: 10.1016/j.cub.2016.04.026

Figure 1. Dissection of Meristematic Cells for Wall Analysis.

Figure 1

(A) Schematic representation of the organization of Arabidopsis shoot apex. Meristematic cells (purple) in both shoot apical meristem (SAM) and flower primordia were collected for analysis. CZ, central zone; PZ, peripheral zone.

(B and C) Schematic flowchart displaying the strategy used in this study. The meristematic cells carefully dissected from clv3-2 SAM and flower primordia (before Stage 6 according to [20]) (C) were used for cell wall preparation and RNA extraction. Cell wall composition was determined by linkage analysis. Glycosyltransferase (GT) gene expression was identified by RNA sequencing (B).

(D) The expression of genes specific to SAM (APUM10 and WUS), flower (AP1 and AG) and shoot vasculature (CESA7, COBL4, TED6 and SND2) in dissected shoot apical meristem and young flower. The shoot sample was included for comparison. Arabidopsis UBIQUITIN10 gene was used as an internal control. Shown are mean values from three replicates, error bars represent standard deviation values.