Table 1.
Performance comparison of FarSight, NeuroGPS, HeY’s method, GFT and the proposed algorithm in different datasets
| Dataset | Type | Volume | Ground truth | Proposed | FarSight | NeuroGPS | HeY’s method | GFT |
|---|---|---|---|---|---|---|---|---|
| Data 1 | 2p-fMOST | 150 × 150 × 150 | 788 | 764/0.92/ | 805/0.85/ | 767/0.78/ | 620/0.70/ | 490/0.60/ |
| 2 × 2 × 2 μm3 | 0.95/0.93a | 0.83/0.84 | 0.80/0.79 | 0.89/0.78 | 0.97/0.74 | |||
| Data 2 | 2p-fMOST | 100 × 100 × 100 | 288 | 280/0.92/ | 268/0.75/ | 265/0.84/ | 246/0.77/ | 231/0.74/ |
| 2 × 2 × 2 μm3 | 0.95/0.93 | 0.81/0.78 | 0.92/0.88 | 0.90/0.83 | 0.92/0.82 | |||
| Data 3 | 2p-fMOST | 100 × 100 × 100 | 25 | 24/0.96/ | 24/0.88/ | 24/0.96/ | 24/0.96/ | 24/0.96/ |
| 2 × 2 × 2 μm3 | 1.00/0.98 | 0.92/0.90 | 1.00/0.98 | 1.00/0.98 | 1.00/0.98 | |||
| Data 4 | 2p-fMOST | 100 × 100 × 100 | 164 | 170/0.94/ | 179/0.76/ | 172/0.88/ | 165/0.82/ | 144/0.73/ |
| 2 × 2 × 2 μm3 | 0.91/0.92 | 0.70/0.73 | 0.84/0.86 | 0.81/0.81 | 0.83/0.77 | |||
| Data 5 | 2p-fMOST | 100 × 100 × 100 | 148 | 147/0.96/ | 173/0.86/ | 146/0.94/ | 147/0.84/ | 135/0.86/ |
| 2 × 2 × 2 μm3 | 0.97/0.96 | 0.74/0.80 | 0.95/0.95 | 0.85/0.85 | 0.94/0.90 | |||
| Data 6 | SIMb | 100 × 100 × 100 | 496 | 486/0.95/ | 528/0.82/ | 407/0.82/ | 399/0.79/ | 452/0.89/ |
| 1 × 1 × 2 μm3 | 0.97/0.96 | 0.77/0.80 | 0.99/0.90 | 0.99/0.88 | 0.98/0.93 | |||
| Data 7 | SIM | 100 × 100 × 100 | 93 | 89/0.96/ | 97/0.88/ | 84/0.90/ | 87/0.90/ | 83/0.88/ |
| 1 × 1 × 2 μm3 | 1.00/0.98 | 0.85/0.86 | 1.00/0.95 | 0.97/0.93 | 0.99/0.93 | |||
| Data 8 | Nissl staining | 100 × 100 × 100 | 695 | 616/0.85/ | 680/0.86/ | 551/0.77/ | 502/0.66/ | 501/0.71/ |
| 1 × 1 × 1 μm3 | 0.95/0.90 | 0.84/0.85 | 0.97/0.86 | 0.91/0.77 | 0.98/0.82 | |||
| Data 9 | Nissl staining | 100 × 100 × 50 | 287 | 266/0.90/ | 263/0.79/ | 216/0.74/ | 197/0.67/ | 187/0.64/ |
| 1 × 1 × 1 μm3 | 0.97/0.93 | 0.86/0.82 | 0.98/0.84 | 0.97/0.79 | 0.99/0.78 | |||
| Mean ± SD | /recall | 0.93 ± 0.04 | 0.83 ± 0.05 | 0.85 ± 0.08 | 0.79 ± 0.10 | 0.78 ± 0.12 | ||
| /precision | 0.96 ± 0.03 | 0.81 ± 0.07 | 0.94 ± 0.07 | 0.92 ± 0.07 | 0.96 ± 0.05 | |||
| /F 1 | 0.94 ± 0.03 | 0.82 ± 0.05 | 0.89 ± 0.06 | 0.85 ± 0.07 | 0.85 ± 0.08 | |||
| two-side p-value of Mann–Whitney test | /recall | 0.001** | 0.021ns | 0.005** | 0.008** | |||
| compared with the proposed method | /precision | 0.000** | 0.893ns | 0.209ns | 0.789ns | |||
| /F 1 | 0.000** | 0.051ns | 0.010** | 0.025* | ||||
ns not significant
*p ≤ 0.05, ** p ≤ 0.01
aNumber of detected cells/recall/precision/F 1-measure
bStructured illumination microscopy