Fig. 6.

ITSN-1s collapses vimentin filaments. Immunofluorescence staining using vimentin/AF594 Ab demonstrate a widespread vimentin filament network in A549 cells (a) and an altered subcellular distribution of vimentin with collapse of vimentin intermediate filaments into clusters of short filaments near the nucleus in A549 + ITSN-1s cells (b). Biochemical analysis and densitometry of full length and collapsed vimentin filaments in A549 and A549 + ITSN-1s cells lysates (c). Data are representative of 3 independent experiments performed under identical experimental conditions. 50 cells per experiment were analyzed. Images were acquired using identical parameters. Bars: 10 μm (a, b). Data are shown as mean ± SE, *p < 0.05