Fig. 8.

ITSN-1s enhances Cbl-Eps8 interaction leading increased ubiquitination of Eps8. a A549 and A549 + ITSN-1s cells pretreated with MG132 (10 μM for 2 h) and untreated cells were lysed and subjected to IP with Eps8 Ab followed by WB with Eps8 and Ub Abs. Arrows and bracket show ubiquitinated Eps8. b Densitometry of Ub-Eps8 expressed as percentage of control. c IP with Cbl Ab followed by WB with Eps8 Ab. The lower panels illustrate the Cbl and Eps8 protein levels in A549 and A549 + ITSN-1s cell lysates. Rabbit IgG was used as control (not shown). d Densitometry of Eps8-Cbl interaction expressed as percentage of control. e A549 and A549 + ITSN-1s cells transfected with Cbl-siRNA#1 and #2 or control-siRNA. Lysates were blotted with Cbl Ab (middle panel) followed by Eps8 Ab (upper panel). Actin was used as loading control (lower panel). Data are shown as mean ± SE. *p < 0.05; All data are representative of 3 independent experiments performed under identical experimental conditions