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. 2016 Sep 15;11:88. doi: 10.1186/s13000-016-0536-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Downregulation of miR-424-5p in ESCC tissues and cell lines. a The expression levels of miR-424-5p were analyzed in ESCC tissues (T) and adjacent normal mucosa tissues (N) by real-time PCR method, RNU6B levels were used as an internal control. And the results of real-time PCR assay revealed down-regulation of miR-424-5p in ESCC tissue compare with adjacent normal mucosa tissues. **p < 0.01. b The expression levels of miR-424-5p were analyzed in ESCC cell lines (EC9706, Eca109 and EC-1) and immortal embryonic esophageal epithelium cell line (SHEE) by real-time PCR method, RNU6B levels were used as an internal control. Expression of miR-424-5p was down-regulated in EC9706, Eca109 and EC-1 compared with SHEE. **p < 0.01. The expression of miR-424-5p in EC-1 was the lowest in three kinds of ESCC cell lines although there had no statistical difference among them