Fig. 2.

Overexperssion of miR-424-5p inhibited EC-1 cells invasion and metastasis. a Real-time PCR analysis of miR-424-5p in EC-1 cells transfected with miR-424-5p mimics showed increased miR-424-5p expression compared with EC-1 cells transfected with those in negative control or blank control. **p < 0.01. b The results of cell invasion assay showed that the average number of invasive cells in miR-424-5p mimics groups were greatly lower than those in negative control or blank control. *p < 0.05. Representative fields of invasive cells on the membrane were shown. c The results of wound healing assay also showed that the wound healing ability of EC-1 cells transfected with miR-424-5p were significantly increased compared with EC-1 cells transfected with those in negative control or blank control. *p < 0.05. Representative pictures of wound healing distance were shown