Table 2.
Number of genes that were expressed in each treatment, as identified by GO categories
| ROS-/NO-related genes | GO category | Gene family | No. of gene up/down regulated | ||
|---|---|---|---|---|---|
| P | HC | PHC | |||
| ROS-related genes | |||||
| ROS-generating genes | GO:0043167 ion binding |
Respiratory burst oxidase homolog protein (VvRBOH) | 0/0 | 0/1 | 0/2 |
| GO:0004601 peroxidase activity |
Peroxidase (VvPOD) | 10/1 | 10/14 | 15/10 | |
| ROS-scavenging genes | GO:0016209 antioxidant activity |
Alpha-dioxygenase 1 (VvDOX1) | 1/0 | 1/0 | 0/0 |
| Glutathione peroxidase (VvGPX) | 0/0 | 0/1 | 1/1 | ||
| Catalase isozyme 1-like (VvCAT) | 0/0 | 0/2 | 0/2 | ||
| Cytosolic ascorbate peroxidase (VvAPX) | 0/0 | 0/1 | 0/2 | ||
| Superoxide dismutase [Fe], chloroplastic (VvFSD) | 0/0 | 0/0 | 1/1 | ||
| Alternative oxidase 2, mitochondrial (VvAOX2) | 0/0 | 1/0 | 1/0 | ||
| ROS-specific transcription factors | GO:00037000 transcription factor |
Heat Stress transcription factor (VvHSF) | 0/1 | 2/2 | 3/6 |
| Ethylene response element binding factor (VvERF) | 4/3 | 8/6 | 8/9 | ||
| WRKY transcription factor (VvWRKY) | 1/0 | 9/4 | 7/7 | ||
| NO-related gene | |||||
| NO-scavenging | GO:0005488 Binding |
Hemoglobin-2 (VvHB2) | 0/0 | 1/0 | 1/0 |
These were significantly up/down regulated genes related to ROS/NO metabolism in grapevine buds treated with P, HC and PHC following the GO categories by ErmineJ analysis