Table 2.
Main metabolites in fermentations run by the parent FX10 strain and recombinant derivatives
1Biomass (DO600 nm) | 2Residual sugars (g/L) | 2Ethanol (% vol/vol) | 2Acetic acid (g/L) | 2Glycerol (g/L) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Anaerobic | Aerobic | Anaerobic | Aerobic | Anaerobic | Aerobic | Anaerobic | Aerobic | Anaerobic | Aerobic | |
FX10 | 13.93 ± 1.93b | 20.93 ± 1.53bc | 0.10 ± 0.17a | 0.03 ± 0.06a | 8.70 ± 0.24c | 5.63 ± 0.35b | 0.14 ± 0.03bc | 3.34 ± 0.48cd | 10.28 ± 0.24a | 5.80 ± 0.20a |
FHXK2 | 14.53 ± 1.52b | 22.70 ± 2.35bc | 0.10 ± 0.17a | 0.03 ± 0.06a | 8.71 ± 0.34c | 5.59 ± 1.01b | 0.16 ± 0.08c | 3.57 ± 0.38d | 11.00 ± 0.17 ab | 5.98 ± 0.03a |
FPYK1 | 10.63 ± 1.67ab | 24.63 ± 1.38c | 0.17 ± 0.21a | 0.06 ± 0.06a | 8.51 ± 0.09c | 5.79 ± 0.21b | 0.11 ± 0.03abc | 4.57 ± 0.89d | 10.52 ± 0.03ab | 6.10 ± 0.20a |
FREG1 | 12.30 ± 0.70ab | 24.72 ± 2.02c | 22.50 ± 5.11b | 38.00 ± 2.17b | 6.70 ± 0.40a | 4.03 ± 0.34a | 0.15 ± 0.04c | 0.15 ± 0.02a | 10.73 ± 1.47ab | 17.57 ± 0.32b |
FPDC1 | 11.71 ± 2.78ab | 18.75 ± 2.19b | ND | ND | 8.19 ± 0.26bc | 5.64 ± 0.37b | 0.06 ± 0.04ab | 0.73 ± 0.47ab | 9.85 ± 0.06a | 5.55 ± 0.44a |
FPDE2 | 7.53 ± 2.05a | 13.13 ± 1.95a | 0.07 ± 0.12a | ND | 7.59 ± 0.25b | 6.49 ± 0.71b | 0.04 ± 0.03a | 1.91 ± 0.76bc | 12.43 ± 0.91b | 6.43 ± 0.67a |
Values are shown as mean ± SD of three biological replicates. The glucose and fructose contents of the natural must in the fermentations were ranged in 190.4–195.8 g/L. Different letters indicate statistically significant differences (HSD Tukey) for values in the same column
ND not detectable
1Analyses were performed after 4 days of fermentation
2Analyses were performed after 7 days of fermentation