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. 2016 Sep 15;15:156. doi: 10.1186/s12934-016-0555-y

Table 2.

Main metabolites in fermentations run by the parent FX10 strain and recombinant derivatives

1Biomass (DO600 nm) 2Residual sugars (g/L) 2Ethanol (% vol/vol) 2Acetic acid (g/L) 2Glycerol (g/L)
Anaerobic Aerobic Anaerobic Aerobic Anaerobic Aerobic Anaerobic Aerobic Anaerobic Aerobic
FX10 13.93 ± 1.93b 20.93 ± 1.53bc 0.10 ± 0.17a 0.03 ± 0.06a 8.70 ± 0.24c 5.63 ± 0.35b 0.14 ± 0.03bc 3.34 ± 0.48cd 10.28 ± 0.24a 5.80 ± 0.20a
FHXK2 14.53 ± 1.52b 22.70 ± 2.35bc 0.10 ± 0.17a 0.03 ± 0.06a 8.71 ± 0.34c 5.59 ± 1.01b 0.16 ± 0.08c 3.57 ± 0.38d 11.00 ± 0.17 ab 5.98 ± 0.03a
FPYK1 10.63 ± 1.67ab 24.63 ± 1.38c 0.17 ± 0.21a 0.06 ± 0.06a 8.51 ± 0.09c 5.79 ± 0.21b 0.11 ± 0.03abc 4.57 ± 0.89d 10.52 ± 0.03ab 6.10 ± 0.20a
FREG1 12.30 ± 0.70ab 24.72 ± 2.02c 22.50 ± 5.11b 38.00 ± 2.17b 6.70 ± 0.40a 4.03 ± 0.34a 0.15 ± 0.04c 0.15 ± 0.02a 10.73 ± 1.47ab 17.57 ± 0.32b
FPDC1 11.71 ± 2.78ab 18.75 ± 2.19b ND ND 8.19 ± 0.26bc 5.64 ± 0.37b 0.06 ± 0.04ab 0.73 ± 0.47ab 9.85 ± 0.06a 5.55 ± 0.44a
FPDE2 7.53 ± 2.05a 13.13 ± 1.95a 0.07 ± 0.12a ND 7.59 ± 0.25b 6.49 ± 0.71b 0.04 ± 0.03a 1.91 ± 0.76bc 12.43 ± 0.91b 6.43 ± 0.67a

Values are shown as mean ± SD of three biological replicates. The glucose and fructose contents of the natural must in the fermentations were ranged in 190.4–195.8 g/L. Different letters indicate statistically significant differences (HSD Tukey) for values in the same column

ND not detectable

1Analyses were performed after 4 days of fermentation

2Analyses were performed after 7 days of fermentation